The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover

Shaowen Ji, Ilsoon Lee

    Research output: ResearchConference contribution

    Abstract

    Lignocellulose is the most abundant renewable biomass and the potential substrate for ethanol production. The costly pretreatments and large consumption of enzymes are needed to reach high cellulose conversion. Lignin, as one of major cell wall components, is considered as the barrier to enzymatic hydrolysis by blocking cellulase from productively being adsorbed. The removal or effective modification of lignin is required for efficient pretreatment process. In this study, the effects of cationic polyelectrolyte, poly-(dimethyldiallylammonium chloride) (PDAC), on alkaline pretreated corn stover were investigated. Structural study by Scanning Electron Microscopy (SEM) imaging revealed particles morphology on cell walls of pretreated corn stover. These particles were confirmed to contain lignin by Confocal Laser Scanning Microscopy (CLSM) and Transmission Electron Microscopy (TEM) imaging. Chemical compositions of pretreated corn stover at different alkaline and polyelectrolyte concentrations were analyzed. Higher polyelectrolyte concentration in the pretreatment resulted in higher lignin retention. Evaluation of enzymatic hydrolysis showed the enhancement of cellulose and hemicellulose conversion after polyelectrolyte pretreatment. PDAC was proposed to facilitate cellulose disruption and lignin redistribution via its interaction with lignin, which significantly modified biomass morphological properties, such as increased porosity and improved accessibility of cellulose surface.

    LanguageEnglish (US)
    Title of host publication11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings
    StatePublished - 2011
    Event2011 AIChE Annual Meeting, 11AIChE - Minneapolis, MN, United States
    Duration: Oct 16 2011Oct 21 2011

    Other

    Other2011 AIChE Annual Meeting, 11AIChE
    CountryUnited States
    CityMinneapolis, MN
    Period10/16/1110/21/11

    Profile

    Enzymatic hydrolysis
    Lignin
    polycations
    Polyelectrolytes
    Cellulose
    Chlorides
    Biomass
    Cells
    Imaging techniques
    Cellulase
    Microscopic examination
    Ethanol
    Porosity
    Transmission electron microscopy
    Scanning
    Scanning electron microscopy
    Lasers
    Substrates
    Enzymes
    Chemical analysis

    ASJC Scopus subject areas

    • Chemical Engineering(all)

    Cite this

    Ji, S., & Lee, I. (2011). The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover. In 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings

    The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover. / Ji, Shaowen; Lee, Ilsoon.

    11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. 2011.

    Research output: ResearchConference contribution

    Ji, S & Lee, I 2011, The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover. in 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. 2011 AIChE Annual Meeting, 11AIChE, Minneapolis, MN, United States, 10/16/11.
    Ji S, Lee I. The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover. In 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. 2011.
    Ji, Shaowen ; Lee, Ilsoon. / The effects of cationic polyelectrolyte on the pretreatment and enzymatic hydrolysis of corn stover. 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. 2011.
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