The role of fatty acid unsaturation in minimizing biophysical changes on the structure and local effects of bilayer membranes

Sukit Leekumjorn, Hyun Ju Cho, Yifei Wu, Neil T. Wright, Amadeu K. Sum, Christina Chan

    Research output: Contribution to journalArticle

    • 25 Citations

    Abstract

    Studying the effects of saturated and unsaturated fatty acids on biological and model (liposomes) membranes could provide insight into the contribution of biophysical effects on the cytotoxicity observed with saturated fatty acids. In vitro experiments suggest that unsaturated fatty acids, such as oleate and linoleate, are less toxic, and have less impact on the membrane fluidity. To understand and assess the biophysical changes in the presence of the different fatty acids, we performed computational analyses of model liposomes with palmitate, oleate, and linoleate. The computational results indicate that the unsaturated fatty acid chain serves as a membrane stabilizer by preventing changes to the membrane fluidity. Based on a Voronoi tessellation analysis, unsaturated fatty acids have structural properties that can reduce the lipid ordering within the model membranes. In addition, hydrogen bond analysis indicates a more uniform level of membrane hydration in the presence of oleate and linoleate as compared to palmitate. Altogether, these observations from the computational studies provide a possible mechanism by which unsaturated fatty acids minimize biophysical changes and protect the cellular membrane and structure. To corroborate our findings, we also performed a liposomal leakage study to assess how the different fatty acids alter the membrane integrity of liposomes. This showed that palmitate, a saturated fatty acid, caused greater destabilization of liposomes (more "leaky") than oleate, an unsaturated fatty acid.

    Original languageEnglish (US)
    Pages (from-to)1508-1516
    Number of pages9
    JournalBiochimica et Biophysica Acta - Biomembranes
    Volume1788
    Issue number7
    DOIs
    StatePublished - Jul 2009

    Profile

    Unsaturated Fatty Acids
    Fatty Acids
    Membranes
    Bronchiolo-Alveolar Adenocarcinoma
    Oleic Acid
    Liposomes
    Palmitates
    Linoleic Acid
    Membrane Fluidity
    Anthralin
    Hyperlipoproteinemia Type II
    Biological Models
    Poisons
    Hydrogen
    Lipids
    In Vitro Techniques
    Common Bile Duct Diseases
    Leghemoglobin
    Ethical Review
    Hydration

    Keywords

    • Cytotoxicity
    • Fatty acid
    • Liposome
    • Membrane fluidity
    • Saturated
    • Unsaturated

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Biophysics
    • Medicine(all)

    Cite this

    The role of fatty acid unsaturation in minimizing biophysical changes on the structure and local effects of bilayer membranes. / Leekumjorn, Sukit; Cho, Hyun Ju; Wu, Yifei; Wright, Neil T.; Sum, Amadeu K.; Chan, Christina.

    In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1788, No. 7, 07.2009, p. 1508-1516.

    Research output: Contribution to journalArticle

    Leekumjorn, Sukit; Cho, Hyun Ju; Wu, Yifei; Wright, Neil T.; Sum, Amadeu K.; Chan, Christina / The role of fatty acid unsaturation in minimizing biophysical changes on the structure and local effects of bilayer membranes.

    In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1788, No. 7, 07.2009, p. 1508-1516.

    Research output: Contribution to journalArticle

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