Theoretical and experimental study of bi-enzyme electrodes with substrate recycling

Neeraj Kohli, Ilsoon Lee, Rudy J. Richardson, Robert M. Worden

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

The range of analytes for which biosensors can be developed can be increased substantially by coupling multiple enzyme activities into reaction pathways. The sensitivity of the biosensors can also be increased (or amplified) dramatically by incorporating a substrate-recycling scheme. For low concentrations of substrate, this paper presents a theoretical model for a bi-enzyme biosensor that achieves signal amplification via substrate recycling. The bi-enzyme electrode was fabricated by co-immobilizing two enzymes: tyrosinase and NEST (neuropathy target esterase domain). The model was validated by assembling the bi-enzyme interface on a rotating disk electrode and measuring the biosensor's response to phenyl valerate substrate under varying rotating speeds. The model can help quantify the influences of mass transport, partition coefficients, enzymes, and electron-transfer kinetics on the metrological characteristics of the bi-enzyme electrode. This information can help optimize the performance of biosensors that use substrate recycling.

LanguageEnglish (US)
Pages104-110
Number of pages7
JournalJournal of Electroanalytical Chemistry
Volume641
Issue number1-2
DOIs
StatePublished - Mar 15 2010

Profile

Enzyme electrodes
Biosensors
Recycling
Enzymes
Substrates
Enzyme kinetics
Monophenol Monooxygenase
Rotating disks
Enzyme activity
Amplification
Mass transfer
Electrodes
Kinetics
Electrons

Keywords

  • Bi-enzyme electrode
  • Electrochemical
  • NEST
  • Signal amplification
  • Substrate recycling
  • Theoretical model

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry

Cite this

Theoretical and experimental study of bi-enzyme electrodes with substrate recycling. / Kohli, Neeraj; Lee, Ilsoon; Richardson, Rudy J.; Worden, Robert M.

In: Journal of Electroanalytical Chemistry, Vol. 641, No. 1-2, 15.03.2010, p. 104-110.

Research output: Contribution to journalArticle

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