Theoretical and experimental study of bi-enzyme electrodes with substrate recycling

Neeraj Kohli, Ilsoon Lee, Rudy J. Richardson, Robert M. Worden

    Research output: Research - peer-reviewArticle

    • 7 Citations

    Abstract

    The range of analytes for which biosensors can be developed can be increased substantially by coupling multiple enzyme activities into reaction pathways. The sensitivity of the biosensors can also be increased (or amplified) dramatically by incorporating a substrate-recycling scheme. For low concentrations of substrate, this paper presents a theoretical model for a bi-enzyme biosensor that achieves signal amplification via substrate recycling. The bi-enzyme electrode was fabricated by co-immobilizing two enzymes: tyrosinase and NEST (neuropathy target esterase domain). The model was validated by assembling the bi-enzyme interface on a rotating disk electrode and measuring the biosensor's response to phenyl valerate substrate under varying rotating speeds. The model can help quantify the influences of mass transport, partition coefficients, enzymes, and electron-transfer kinetics on the metrological characteristics of the bi-enzyme electrode. This information can help optimize the performance of biosensors that use substrate recycling.

    LanguageEnglish (US)
    Pages104-110
    Number of pages7
    JournalJournal of Electroanalytical Chemistry
    Volume641
    Issue number1-2
    DOIs
    StatePublished - Mar 15 2010

    Profile

    Enzyme electrodes
    Biosensors
    Recycling
    Substrates
    Enzymes
    Monophenol Monooxygenase
    Rotating disks
    Enzyme activity
    Amplification
    Mass transfer
    Electrodes
    Kinetics
    Electrons
    phenyl valerate
    neurotoxic esterase
    Enzyme kinetics
    Esterases

    Keywords

    • Bi-enzyme electrode
    • Electrochemical
    • NEST
    • Signal amplification
    • Substrate recycling
    • Theoretical model

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Analytical Chemistry
    • Electrochemistry

    Cite this

    Theoretical and experimental study of bi-enzyme electrodes with substrate recycling. / Kohli, Neeraj; Lee, Ilsoon; Richardson, Rudy J.; Worden, Robert M.

    In: Journal of Electroanalytical Chemistry, Vol. 641, No. 1-2, 15.03.2010, p. 104-110.

    Research output: Research - peer-reviewArticle

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