Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels

Sumit Mehrotra, Daniel Lynam, Ryan Maloney, Kendell M. Pawelec, Mark H. Tuszynski, Ilsoon Lee, Christina Chan, Jeffrey Sakamoto

    Research output: Contribution to journalArticle

    • 65 Citations

    Abstract

    Axons of the adult central nervous system exhibit an extremely limited ability to regenerate after spinal cord injury. Experimentally generated patterns of axon growth are typically disorganized and randomly oriented. Support of linear axonal growth into spinal cord lesion sites has been demonstrated using arrays of uniaxial channels, templated with agarose hydrogel, and containing genetically engineered cells that secrete brain-derived neurotrophic factor (8DNF). However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Existing methods of loading the drug or protein into hydrogels cannot provide sustained release from templated aprose hydrogels. Alternatively, here it is shown that pH-responsive H-bonded poly(ethylene glycoI)(PEG)/poly{acrylic acid)(PAA)/protein hybrid layer-bylayer (LbL) thin films, when prepared over agarose, provided sustained release of protein under physiological conditions for more than four weeks. Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. This is the first demonstration of month-long sustained protein release from an agarose hydrogel, whereby the drug/protein is loaded separately from the agarose hydrogel fabrication process.

    Original languageEnglish (US)
    Pages (from-to)247-258
    Number of pages12
    JournalAdvanced Functional Materials
    Volume20
    Issue number2
    DOIs
    StatePublished - Jan 22 2010

    Profile

    Proteins
    proteins
    Hydrogels
    Multilayers
    axons
    drugs
    cells
    Erythrasma
    Micelles
    Scaffolds
    Axons
    Neurology
    Scaffolds (biology)
    Polyethylene glycols
    Acrylics
    Brain
    Ethylene
    Demonstrations
    Enzymes
    Fabrication

    ASJC Scopus subject areas

    • Biomaterials
    • Electrochemistry
    • Condensed Matter Physics
    • Electronic, Optical and Magnetic Materials

    Cite this

    Mehrotra, S., Lynam, D., Maloney, R., Pawelec, K. M., Tuszynski, M. H., Lee, I., ... Sakamoto, J. (2010). Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels. Advanced Functional Materials, 20(2), 247-258. DOI: 10.1002/adfm.200901172

    Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels. / Mehrotra, Sumit; Lynam, Daniel; Maloney, Ryan; Pawelec, Kendell M.; Tuszynski, Mark H.; Lee, Ilsoon; Chan, Christina; Sakamoto, Jeffrey.

    In: Advanced Functional Materials, Vol. 20, No. 2, 22.01.2010, p. 247-258.

    Research output: Contribution to journalArticle

    Mehrotra, S, Lynam, D, Maloney, R, Pawelec, KM, Tuszynski, MH, Lee, I, Chan, C & Sakamoto, J 2010, 'Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels' Advanced Functional Materials, vol 20, no. 2, pp. 247-258. DOI: 10.1002/adfm.200901172
    Mehrotra S, Lynam D, Maloney R, Pawelec KM, Tuszynski MH, Lee I et al. Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels. Advanced Functional Materials. 2010 Jan 22;20(2):247-258. Available from, DOI: 10.1002/adfm.200901172

    Mehrotra, Sumit; Lynam, Daniel; Maloney, Ryan; Pawelec, Kendell M.; Tuszynski, Mark H.; Lee, Ilsoon; Chan, Christina; Sakamoto, Jeffrey / Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels.

    In: Advanced Functional Materials, Vol. 20, No. 2, 22.01.2010, p. 247-258.

    Research output: Contribution to journalArticle

    @article{30433b7cfec74a29b11afa0b8646513c,
    title = "Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels",
    abstract = "Axons of the adult central nervous system exhibit an extremely limited ability to regenerate after spinal cord injury. Experimentally generated patterns of axon growth are typically disorganized and randomly oriented. Support of linear axonal growth into spinal cord lesion sites has been demonstrated using arrays of uniaxial channels, templated with agarose hydrogel, and containing genetically engineered cells that secrete brain-derived neurotrophic factor (8DNF). However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Existing methods of loading the drug or protein into hydrogels cannot provide sustained release from templated aprose hydrogels. Alternatively, here it is shown that pH-responsive H-bonded poly(ethylene glycoI)(PEG)/poly{acrylic acid)(PAA)/protein hybrid layer-bylayer (LbL) thin films, when prepared over agarose, provided sustained release of protein under physiological conditions for more than four weeks. Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. This is the first demonstration of month-long sustained protein release from an agarose hydrogel, whereby the drug/protein is loaded separately from the agarose hydrogel fabrication process.",
    author = "Sumit Mehrotra and Daniel Lynam and Ryan Maloney and Pawelec, {Kendell M.} and Tuszynski, {Mark H.} and Ilsoon Lee and Christina Chan and Jeffrey Sakamoto",
    year = "2010",
    month = "1",
    doi = "10.1002/adfm.200901172",
    volume = "20",
    pages = "247--258",
    journal = "Advanced Functional Materials",
    issn = "1616-301X",
    publisher = "Wiley-VCH Verlag",
    number = "2",

    }

    TY - JOUR

    T1 - Time controlled protein release from layer-by-layer assembled multilayer functionalized agarose hydrogels

    AU - Mehrotra,Sumit

    AU - Lynam,Daniel

    AU - Maloney,Ryan

    AU - Pawelec,Kendell M.

    AU - Tuszynski,Mark H.

    AU - Lee,Ilsoon

    AU - Chan,Christina

    AU - Sakamoto,Jeffrey

    PY - 2010/1/22

    Y1 - 2010/1/22

    N2 - Axons of the adult central nervous system exhibit an extremely limited ability to regenerate after spinal cord injury. Experimentally generated patterns of axon growth are typically disorganized and randomly oriented. Support of linear axonal growth into spinal cord lesion sites has been demonstrated using arrays of uniaxial channels, templated with agarose hydrogel, and containing genetically engineered cells that secrete brain-derived neurotrophic factor (8DNF). However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Existing methods of loading the drug or protein into hydrogels cannot provide sustained release from templated aprose hydrogels. Alternatively, here it is shown that pH-responsive H-bonded poly(ethylene glycoI)(PEG)/poly{acrylic acid)(PAA)/protein hybrid layer-bylayer (LbL) thin films, when prepared over agarose, provided sustained release of protein under physiological conditions for more than four weeks. Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. This is the first demonstration of month-long sustained protein release from an agarose hydrogel, whereby the drug/protein is loaded separately from the agarose hydrogel fabrication process.

    AB - Axons of the adult central nervous system exhibit an extremely limited ability to regenerate after spinal cord injury. Experimentally generated patterns of axon growth are typically disorganized and randomly oriented. Support of linear axonal growth into spinal cord lesion sites has been demonstrated using arrays of uniaxial channels, templated with agarose hydrogel, and containing genetically engineered cells that secrete brain-derived neurotrophic factor (8DNF). However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Existing methods of loading the drug or protein into hydrogels cannot provide sustained release from templated aprose hydrogels. Alternatively, here it is shown that pH-responsive H-bonded poly(ethylene glycoI)(PEG)/poly{acrylic acid)(PAA)/protein hybrid layer-bylayer (LbL) thin films, when prepared over agarose, provided sustained release of protein under physiological conditions for more than four weeks. Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. This is the first demonstration of month-long sustained protein release from an agarose hydrogel, whereby the drug/protein is loaded separately from the agarose hydrogel fabrication process.

    UR - http://www.scopus.com/inward/record.url?scp=74349083656&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=74349083656&partnerID=8YFLogxK

    U2 - 10.1002/adfm.200901172

    DO - 10.1002/adfm.200901172

    M3 - Article

    VL - 20

    SP - 247

    EP - 258

    JO - Advanced Functional Materials

    T2 - Advanced Functional Materials

    JF - Advanced Functional Materials

    SN - 1616-301X

    IS - 2

    ER -