Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly: An in vitro study

Sumit Mehrotra, Daniel Lynam, Chun Liu, Dena Shahriari, Ilsoon Lee, Mark Tuszynski, Jeffrey Sakamoto, Christina Chan

Research output: Contribution to journalArticle

  • 16 Citations

Abstract

Experimentally induced axonal regeneration is compromised by glial scar formation arising from leptomeningeal fibroblasts cells in and around the hydrogel scaffold implanted for nerve repair. Strategies are needed to prevent such fibroblastic reactive cell layer formation for enhanced axonal regeneration. Here, we implement the technique of layer-by-layer assembled degradable, hydrogen bonded multilayers on agarose hydrogels to incorporate an anti-mitotic drug (1-β-D-arabinofuranosylcytosine (Ara-C)) within the agarose hydrogels. We show controlled release of Ara-C under physiological conditions over a period of days. The concentrations of Ara-C released from agarose at the different time points were sufficient to inhibit fibroblast growth in vitro, while not adversely affecting the viability of the neuronal cells.

LanguageEnglish (US)
Pages439-463
Number of pages25
JournalJournal of Biomaterials Science, Polymer Edition
Volume23
Issue number1-4
DOIs
StatePublished - 2012

Profile

Hydrogels
Cytarabine
Sepharose
Cells
Fibroblasts
Regeneration
Scaffolds
Hydrogel
Multilayers
Repair
Neuroglia
Cicatrix
Hydrogen
Cell Survival
In Vitro Techniques
Growth
Pharmaceutical Preparations

Keywords

  • agarose
  • Ara-C
  • layer-by-layer assembly
  • nerve repair
  • reactive cell layer
  • Time controlled release

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly : An in vitro study. / Mehrotra, Sumit; Lynam, Daniel; Liu, Chun; Shahriari, Dena; Lee, Ilsoon; Tuszynski, Mark; Sakamoto, Jeffrey; Chan, Christina.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 1-4, 2012, p. 439-463.

Research output: Contribution to journalArticle

Mehrotra, Sumit ; Lynam, Daniel ; Liu, Chun ; Shahriari, Dena ; Lee, Ilsoon ; Tuszynski, Mark ; Sakamoto, Jeffrey ; Chan, Christina. / Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly : An in vitro study. In: Journal of Biomaterials Science, Polymer Edition. 2012 ; Vol. 23, No. 1-4. pp. 439-463
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