Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly: An in vitro study

Sumit Mehrotra, Daniel Lynam, Chun Liu, Dena Shahriari, Ilsoon Lee, Mark Tuszynski, Jeffrey Sakamoto, Christina Chan

    Research output: Contribution to journalArticle

    • 14 Citations

    Abstract

    Experimentally induced axonal regeneration is compromised by glial scar formation arising from leptomeningeal fibroblasts cells in and around the hydrogel scaffold implanted for nerve repair. Strategies are needed to prevent such fibroblastic reactive cell layer formation for enhanced axonal regeneration. Here, we implement the technique of layer-by-layer assembled degradable, hydrogen bonded multilayers on agarose hydrogels to incorporate an anti-mitotic drug (1-β-D-arabinofuranosylcytosine (Ara-C)) within the agarose hydrogels. We show controlled release of Ara-C under physiological conditions over a period of days. The concentrations of Ara-C released from agarose at the different time points were sufficient to inhibit fibroblast growth in vitro, while not adversely affecting the viability of the neuronal cells.

    Original languageEnglish (US)
    Pages (from-to)439-463
    Number of pages25
    JournalJournal of Biomaterials Science, Polymer Edition
    Volume23
    Issue number1-4
    DOIs
    StatePublished - 2012

    Profile

    Hydrogels
    Cytarabine
    Sepharose
    In Vitro Techniques
    Cells
    Castration
    Regeneration
    Fibroblasts
    Hydrogel
    Neuroglia
    Cicatrix
    Hydrogen
    Cell Survival
    Scaffolds
    Multilayers
    Repair
    Erythrasma
    Micelles

    Keywords

    • agarose
    • Ara-C
    • layer-by-layer assembly
    • nerve repair
    • reactive cell layer
    • Time controlled release

    ASJC Scopus subject areas

    • Biophysics
    • Biomaterials
    • Bioengineering
    • Biomedical Engineering

    Cite this

    Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly : An in vitro study. / Mehrotra, Sumit; Lynam, Daniel; Liu, Chun; Shahriari, Dena; Lee, Ilsoon; Tuszynski, Mark; Sakamoto, Jeffrey; Chan, Christina.

    In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 1-4, 2012, p. 439-463.

    Research output: Contribution to journalArticle

    Mehrotra, Sumit; Lynam, Daniel; Liu, Chun; Shahriari, Dena; Lee, Ilsoon; Tuszynski, Mark; Sakamoto, Jeffrey; Chan, Christina / Time controlled release of arabinofuranosylcytosine (Ara-C) from agarose hydrogels using layer-by-layer assembly : An in vitro study.

    In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 1-4, 2012, p. 439-463.

    Research output: Contribution to journalArticle

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    AU - Tuszynski,Mark

    AU - Sakamoto,Jeffrey

    AU - Chan,Christina

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