Abstract
High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX™ (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST). Decreased sugar conversion and ethanol yield with increasing solids loading were also observed. End-product (ethanol) was proven to be the major cause of this issue and increased degradation products with increasing solids loading was also a cause. For the first time, we show that with in situ removal of end-product by performing SSCF aerobically, sugar conversion stopped decreasing with increasing solids loading and monomeric sugar conversion reached as high as 93% at a high solids loading of 24.9 wt%. Techno-economic analysis was employed to explore the economic possibilities of cellulosic ethanol production at high solids loadings. The results suggest that low-cost in situ removal of ethanol during SSCF would significantly improve the economics of high solids loading processes. Biotechnol. Bioeng. 2017;114: 980–989.
| Language | English (US) |
|---|---|
| Pages | 980-989 |
| Number of pages | 10 |
| Journal | Biotechnology and Bioengineering |
| Volume | 114 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1 2017 |
Profile
Keywords
- AFEX
- AFEX is a trademark of MBI
- corn stover
- enzymatic hydrolysis
- ethanol
- lansing
- Michigan
- solids loading
- SSCF
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Toward high solids loading process for lignocellulosic biofuel production at a low cost. / Jin, Mingjie; Sarks, Cory; Bals, Bryan D.; Posawatz, Nick; Gunawan, Christa; Dale, Bruce E.; Balan, Venkatesh.
In: Biotechnology and Bioengineering, Vol. 114, No. 5, 01.05.2017, p. 980-989.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toward high solids loading process for lignocellulosic biofuel production at a low cost
AU - Jin,Mingjie
AU - Sarks,Cory
AU - Bals,Bryan D.
AU - Posawatz,Nick
AU - Gunawan,Christa
AU - Dale,Bruce E.
AU - Balan,Venkatesh
PY - 2017/5/1
Y1 - 2017/5/1
N2 - High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX™ (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST). Decreased sugar conversion and ethanol yield with increasing solids loading were also observed. End-product (ethanol) was proven to be the major cause of this issue and increased degradation products with increasing solids loading was also a cause. For the first time, we show that with in situ removal of end-product by performing SSCF aerobically, sugar conversion stopped decreasing with increasing solids loading and monomeric sugar conversion reached as high as 93% at a high solids loading of 24.9 wt%. Techno-economic analysis was employed to explore the economic possibilities of cellulosic ethanol production at high solids loadings. The results suggest that low-cost in situ removal of ethanol during SSCF would significantly improve the economics of high solids loading processes. Biotechnol. Bioeng. 2017;114: 980–989.
AB - High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX™ (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST). Decreased sugar conversion and ethanol yield with increasing solids loading were also observed. End-product (ethanol) was proven to be the major cause of this issue and increased degradation products with increasing solids loading was also a cause. For the first time, we show that with in situ removal of end-product by performing SSCF aerobically, sugar conversion stopped decreasing with increasing solids loading and monomeric sugar conversion reached as high as 93% at a high solids loading of 24.9 wt%. Techno-economic analysis was employed to explore the economic possibilities of cellulosic ethanol production at high solids loadings. The results suggest that low-cost in situ removal of ethanol during SSCF would significantly improve the economics of high solids loading processes. Biotechnol. Bioeng. 2017;114: 980–989.
KW - AFEX
KW - AFEX is a trademark of MBI
KW - corn stover
KW - enzymatic hydrolysis
KW - ethanol
KW - lansing
KW - Michigan
KW - solids loading
KW - SSCF
UR - http://www.scopus.com/inward/record.url?scp=85015861763&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015861763&partnerID=8YFLogxK
U2 - 10.1002/bit.26229
DO - 10.1002/bit.26229
M3 - Article
VL - 114
SP - 980
EP - 989
JO - Biotechnology and Bioengineering
T2 - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 5
ER -