Toward high solids loading process for lignocellulosic biofuel production at a low cost

Mingjie Jin, Cory Sarks, Bryan D. Bals, Nick Posawatz, Christa Gunawan, Bruce E. Dale, Venkatesh Balan

    Research output: Contribution to journalArticle

    • 4 Citations

    Abstract

    High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX™ (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST). Decreased sugar conversion and ethanol yield with increasing solids loading were also observed. End-product (ethanol) was proven to be the major cause of this issue and increased degradation products with increasing solids loading was also a cause. For the first time, we show that with in situ removal of end-product by performing SSCF aerobically, sugar conversion stopped decreasing with increasing solids loading and monomeric sugar conversion reached as high as 93% at a high solids loading of 24.9 wt%. Techno-economic analysis was employed to explore the economic possibilities of cellulosic ethanol production at high solids loadings. The results suggest that low-cost in situ removal of ethanol during SSCF would significantly improve the economics of high solids loading processes. Biotechnol. Bioeng. 2017;114: 980–989.

    Original languageEnglish (US)
    Pages (from-to)980-989
    Number of pages10
    JournalBiotechnology and Bioengineering
    Volume114
    Issue number5
    DOIs
    StatePublished - May 1 2017

    Profile

    Biofuels
    Ethanol
    Costs and Cost Analysis
    Butylene Glycols
    Carbohydrates
    Fermentation
    Economics
    Sugars
    Saccharification
    Costs
    Avian Crop
    Removal
    Transmissible Enteritis of Turkeys
    Xylose
    Ammonia
    Zea mays
    Saccharomyces cerevisiae
    Hydrolysis
    Cellulosic ethanol
    Enzymatic hydrolysis

    Keywords

    • AFEX
    • AFEX is a trademark of MBI
    • corn stover
    • enzymatic hydrolysis
    • ethanol
    • lansing
    • Michigan
    • solids loading
    • SSCF

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Toward high solids loading process for lignocellulosic biofuel production at a low cost. / Jin, Mingjie; Sarks, Cory; Bals, Bryan D.; Posawatz, Nick; Gunawan, Christa; Dale, Bruce E.; Balan, Venkatesh.

    In: Biotechnology and Bioengineering, Vol. 114, No. 5, 01.05.2017, p. 980-989.

    Research output: Contribution to journalArticle

    Jin M, Sarks C, Bals BD, Posawatz N, Gunawan C, Dale BE et al. Toward high solids loading process for lignocellulosic biofuel production at a low cost. Biotechnology and Bioengineering. 2017 May 1;114(5):980-989. Available from, DOI: 10.1002/bit.26229

    Jin, Mingjie; Sarks, Cory; Bals, Bryan D.; Posawatz, Nick; Gunawan, Christa; Dale, Bruce E.; Balan, Venkatesh / Toward high solids loading process for lignocellulosic biofuel production at a low cost.

    In: Biotechnology and Bioengineering, Vol. 114, No. 5, 01.05.2017, p. 980-989.

    Research output: Contribution to journalArticle

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