Toward high solids loading process for lignocellulosic biofuel production at a low cost

Mingjie Jin, Cory Sarks, Bryan D. Bals, Nick Posawatz, Christa Gunawan, Bruce E. Dale, Venkatesh Balan

Research output: Contribution to journalArticle

  • 10 Citations

Abstract

High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX™ (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST). Decreased sugar conversion and ethanol yield with increasing solids loading were also observed. End-product (ethanol) was proven to be the major cause of this issue and increased degradation products with increasing solids loading was also a cause. For the first time, we show that with in situ removal of end-product by performing SSCF aerobically, sugar conversion stopped decreasing with increasing solids loading and monomeric sugar conversion reached as high as 93% at a high solids loading of 24.9 wt%. Techno-economic analysis was employed to explore the economic possibilities of cellulosic ethanol production at high solids loadings. The results suggest that low-cost in situ removal of ethanol during SSCF would significantly improve the economics of high solids loading processes. Biotechnol. Bioeng. 2017;114: 980–989.

LanguageEnglish (US)
Pages980-989
Number of pages10
JournalBiotechnology and Bioengineering
Volume114
Issue number5
DOIs
StatePublished - May 1 2017

Profile

Biofuels
Ethanol
Costs and Cost Analysis
Fermentation
Sugars
Costs
Saccharification
Economics
Xylose
Ammonia
Zea mays
Saccharomyces cerevisiae
Cellulosic ethanol
Hydrolysis
Enzymatic hydrolysis
Economic analysis
Yeast
Degradation

Keywords

  • AFEX
  • AFEX is a trademark of MBI
  • corn stover
  • enzymatic hydrolysis
  • ethanol
  • lansing
  • Michigan
  • solids loading
  • SSCF

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Jin, M., Sarks, C., Bals, B. D., Posawatz, N., Gunawan, C., Dale, B. E., & Balan, V. (2017). Toward high solids loading process for lignocellulosic biofuel production at a low cost. Biotechnology and Bioengineering, 114(5), 980-989. DOI: 10.1002/bit.26229

Toward high solids loading process for lignocellulosic biofuel production at a low cost. / Jin, Mingjie; Sarks, Cory; Bals, Bryan D.; Posawatz, Nick; Gunawan, Christa; Dale, Bruce E.; Balan, Venkatesh.

In: Biotechnology and Bioengineering, Vol. 114, No. 5, 01.05.2017, p. 980-989.

Research output: Contribution to journalArticle

Jin, M, Sarks, C, Bals, BD, Posawatz, N, Gunawan, C, Dale, BE & Balan, V 2017, 'Toward high solids loading process for lignocellulosic biofuel production at a low cost' Biotechnology and Bioengineering, vol 114, no. 5, pp. 980-989. DOI: 10.1002/bit.26229
Jin M, Sarks C, Bals BD, Posawatz N, Gunawan C, Dale BE et al. Toward high solids loading process for lignocellulosic biofuel production at a low cost. Biotechnology and Bioengineering. 2017 May 1;114(5):980-989. Available from, DOI: 10.1002/bit.26229
Jin, Mingjie ; Sarks, Cory ; Bals, Bryan D. ; Posawatz, Nick ; Gunawan, Christa ; Dale, Bruce E. ; Balan, Venkatesh. / Toward high solids loading process for lignocellulosic biofuel production at a low cost. In: Biotechnology and Bioengineering. 2017 ; Vol. 114, No. 5. pp. 980-989
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