Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock

Boonchai B. Boonyaratanakornkit, Anjana J. Simpson, Timothy A. Whitehead, Claire M. Fraser, Najib M A El-Sayed, Douglas S. Clark

Research output: Research - peer-reviewArticle

  • 35 Citations

Abstract

Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85°C to 65°C and 95°C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95°C included upregulation of genes encoding chaperones, and down regulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an α subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65°C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPlase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.

LanguageEnglish (US)
Pages789-797
Number of pages9
JournalEnvironmental Microbiology
Volume7
Issue number6
DOIs
StatePublished - Jun 2005
Externally publishedYes

Profile

Methanococcus
cold stress
lethal genes
heat
genes
Methanocaldococcus
Shock
Hot Temperature
Genes
gene
cold
temperature
Temperature
protein
Up-Regulation
heat stress
proteins
Proteins
heat shock
RNA helicases

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. / Boonyaratanakornkit, Boonchai B.; Simpson, Anjana J.; Whitehead, Timothy A.; Fraser, Claire M.; El-Sayed, Najib M A; Clark, Douglas S.

In: Environmental Microbiology, Vol. 7, No. 6, 06.2005, p. 789-797.

Research output: Research - peer-reviewArticle

Boonyaratanakornkit, Boonchai B. ; Simpson, Anjana J. ; Whitehead, Timothy A. ; Fraser, Claire M. ; El-Sayed, Najib M A ; Clark, Douglas S./ Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. In: Environmental Microbiology. 2005 ; Vol. 7, No. 6. pp. 789-797
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