Use of Raman spectroscopy for in situ monitoring of lysozyme concentration during crystallization in a hanging drop

Albert M. Schwartz, Kris A. Berglund

    Research output: Research - peer-reviewArticle

    • 37 Citations

    Abstract

    Fiber optic Raman spectroscopy combined with a partial least-squares regression model was investigated as a means to monitor lysozyme concentration during crystallization in a hanging drop experiment in real time. Raman spectral features of the buffer and protein were employed to build the regression model. This model was used to calculate the compositional changes within the hanging drop. The use of fibre optic technology coupled with Raman spectroscopy, which is ideal for use with aqueous media, results in a powerful noninvasive probe of the changing environment within the solution. These preliminary findings indicate that solubility as well as supersaturation measurements can be made.

    LanguageEnglish (US)
    Pages599-603
    Number of pages5
    JournalJournal of Crystal Growth
    Volume203
    Issue number4
    DOIs
    StatePublished - Jun 1999

    Profile

    Muramidase
    Crystallization
    Raman spectroscopy
    Monitoring
    lysozyme
    crystallization
    Enzymes
    Fiber optics
    regression analysis
    fiber optics
    Supersaturation
    Buffers
    Solubility
    Proteins
    Experiments
    supersaturation
    solubility
    buffers
    proteins
    probes

    ASJC Scopus subject areas

    • Condensed Matter Physics

    Cite this

    Use of Raman spectroscopy for in situ monitoring of lysozyme concentration during crystallization in a hanging drop. / Schwartz, Albert M.; Berglund, Kris A.

    In: Journal of Crystal Growth, Vol. 203, No. 4, 06.1999, p. 599-603.

    Research output: Research - peer-reviewArticle

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