Use of Raman spectroscopy for in situ monitoring of lysozyme concentration during crystallization in a hanging drop

Albert M. Schwartz, Kris A. Berglund

Research output: Contribution to journalArticle

  • 37 Citations

Abstract

Fiber optic Raman spectroscopy combined with a partial least-squares regression model was investigated as a means to monitor lysozyme concentration during crystallization in a hanging drop experiment in real time. Raman spectral features of the buffer and protein were employed to build the regression model. This model was used to calculate the compositional changes within the hanging drop. The use of fibre optic technology coupled with Raman spectroscopy, which is ideal for use with aqueous media, results in a powerful noninvasive probe of the changing environment within the solution. These preliminary findings indicate that solubility as well as supersaturation measurements can be made.

LanguageEnglish (US)
Pages599-603
Number of pages5
JournalJournal of Crystal Growth
Volume203
Issue number4
DOIs
StatePublished - Jun 1999

Profile

lysozyme
Muramidase
Crystallization
Raman spectroscopy
Enzymes
crystallization
Fiber optics
Monitoring
regression analysis
fiber optics
Supersaturation
supersaturation
Buffers
solubility
Solubility
buffers
proteins
Proteins
probes
Experiments

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Use of Raman spectroscopy for in situ monitoring of lysozyme concentration during crystallization in a hanging drop. / Schwartz, Albert M.; Berglund, Kris A.

In: Journal of Crystal Growth, Vol. 203, No. 4, 06.1999, p. 599-603.

Research output: Contribution to journalArticle

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