Versatile bioelectronic interfaces on flexible non-conductive substrates

Brian L. Hassler, Ted J. Amundsen, J. Gregory Zeikus, Ilsoon Lee, Robert M. Worden

    Research output: Contribution to journalArticle

    • 10 Citations

    Abstract

    Bioelectronic interfaces that establish electrical communication between redox enzymes and electrodes have potential applications as biosensors, biocatalytic reactors, and biological fuel cells. These interfaces are commonly formed on gold films deposited using physical vapor deposition (PVD) or chemical vapor deposition (CVD). PVD and CVD require deposition of a primer layer, such as titanium or chromium, and require the use of expensive equipment and cannot be used on a wide range of substrates. This paper describes a versatile new bench-top method to form bioelectronic interfaces containing a gold film, electron mediator, cofactor, and dehydrogenase enzyme (secondary alcohol dehydrogenase, and sorbitol dehydrogenase) on nonconductive substrates such as polystyrene and glass. The method combines layer-by-layer deposition of polyelectrolytes, electroless metal deposition, and directed molecular self-assembly. Cyclic voltammetry, chronoamperometry, field emission X-ray dispersive spectroscopy, scanning electron microscopy, and atomic force microscopy were used to characterize the bioelectronic interfaces. Interfaces formed on flexible polystyrene slides were shown to retain their activity after bending to a radius of curvature of 18 mm, confirming that the approach can be applied on cheap and flexible substrates for applications where traditional wafer-scale electronics is not suitable, such as personal or structural health monitors and rolled microtube biosensors.

    Original languageEnglish (US)
    Pages (from-to)1481-1487
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume23
    Issue number10
    DOIs
    StatePublished - May 15 2008

    Profile

    Substrates
    Biosensing Techniques
    Psychologic Desensitization
    Polystyrenes
    Gold
    Physical vapor deposition
    Biosensors
    Chemical vapor deposition
    Enzymes
    Arthroscopy
    L-Iditol 2-Dehydrogenase
    X-Ray Emission Spectrometry
    Alcohol Dehydrogenase
    Atomic Force Microscopy
    Coenzymes
    Chromium
    Titanium
    Electron Scanning Microscopy
    Oxidation-Reduction
    Glass

    Keywords

    • Bioelectronic interface
    • Biosensor
    • Dehydrogenase
    • Electroless deposition
    • Flexible
    • Plastic

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Electrochemistry

    Cite this

    Versatile bioelectronic interfaces on flexible non-conductive substrates. / Hassler, Brian L.; Amundsen, Ted J.; Zeikus, J. Gregory; Lee, Ilsoon; Worden, Robert M.

    In: Biosensors and Bioelectronics, Vol. 23, No. 10, 15.05.2008, p. 1481-1487.

    Research output: Contribution to journalArticle

    Hassler, Brian L.; Amundsen, Ted J.; Zeikus, J. Gregory; Lee, Ilsoon; Worden, Robert M. / Versatile bioelectronic interfaces on flexible non-conductive substrates.

    In: Biosensors and Bioelectronics, Vol. 23, No. 10, 15.05.2008, p. 1481-1487.

    Research output: Contribution to journalArticle

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